In a current research printed within the journal Frontiers in Vitamin, researchers in contrast the efficacy of mussel and fish oils in stopping atherosclerosis in apolipoprotein E-null (ApoE−/−) mice. Their findings revealed that mussel oil (MO) supplementation over 12 weeks considerably outperformed fish oil (FO) in stopping atherosclerotic plaque buildup within the aorta. MO-supplemented mice additional introduced decrease lipid deposition, macrophage (within the aortic sinus) contents, and easy muscle cell (SMC) contents than their FO-supplemented counterparts. Analyses of MO and FO results on the aorta counsel that MO’s efficiency could also be resulting from its capacity to downregulate the p38MAPK/NF-κB signaling pathway.
Research: Mussel oil is superior to fish oil in stopping atherosclerosis of ApoE−/− mice. Picture Credit score: oksana2010 / Shutterstock
Atherosclerosis and the advantages of PUFA
Atherosclerosis is the buildup of fat, ldl cholesterol, and different substances in and on the artery partitions. This buildup, known as plaque, leads to the thickening or hardening of the arteries, considerably growing the chance of subsequent coronary artery illness, peripheral arterial illness, and carotid artery illness. Analysis has revealed that the crucial mechanisms underpinning the initiation of atherosclerosis are persistent irritation and perturbations in regular lipid metabolism.
The previous a long time have witnessed growing scientific curiosity within the potential of purposeful lipids as interventions towards atherosclerosis. n-3 polyunsaturated fatty acid (n-3 PUFA)-enriched oils, primarily fish oils (FOs), have obtained essentially the most consideration, with research discovering that low-density lipoprotein (LDL) receptor knock-out mice fed with FOs obtained considerably decrease atherosclerotic lesion areas than their placebo-fed counterparts. The apolipoprotein E-null (ApoE−/−) mice mannequin is an especially well-liked murine consultant of human lesions. Research utilizing this in vivo system have proven that n-3 PUFA consumption reduces the chance of carotid intima-media thickness.
The mechanism of motion is attributed to n-3 PUFA’s function in bettering lipid metabolism and its anti-inflammatory properties. One other largely ignored n-3 PUFA supply is mussel oil (MO). Earlier publications by the current analysis group have revealed that MO improved glycaemic traits in each murine and human cohorts. Surprisingly, each this analysis group and others have noticed MOs to have larger efficacy than FOs whereas additional displaying potent anti-inflammatory properties in treating rheumatoid arthritis, sort 2 diabetes mellitus (T2DM), and extra serum triacylglycerol (TG).
Given these advantages and the environmentally sustainable farming potential introduced by mussels, the current research goals to analyze if MOs’ anti-atherosclerosis efficiency matches or exceeds that of their fish-derived counterparts.
Concerning the research
Mussel oil to be used on this research was obtained utilizing lyophilization adopted by supercritical fluid extraction. FO and CO had been bought from licensed distributors. The pattern cohort comprised six-week-old male C57BL/6 J mice (wild sort [WT]; n = 6) and male ApoE−/− C57BL/6 J mice (n = 24) of the identical age. Following one week of acclimatization, the ApoE−/− cohort was randomly assigned to one in all 4 experimental interventions – corn oil (CO), FO, MO, or aspirin (ASP; 0.5 mg/mL) dissolved in CO. Their feed was excessive in fats and ldl cholesterol (HFHC). The WT cohort represented the well being management group, whose remedy was an equal quantity of CO (0.1 mL/10 g/day) and fed a normal chow food plan.
Research investigations included histologic evaluation and atherosclerotic plaque quantitation through a mixture of staining (hematoxylin-eosin and Oil-red O), microscopy, and cryotomy strategies. Immunohistochemistry was used to detect macrophages and easy muscle cells (SMCs) belonging to the aortic sinus. Moreover, fatty acids, serum lipids, and inflammatory components had been quantified.
Western blotting analyses had been used to find out aortal gene protein ranges. Extremely-performance liquid chromatography-mass spectrometry (UPLC-MS) strategies had been used to analyze portions of furan fatty acids and high-performance liquid chromatography (HPLC) to quantify astaxanthin. Between-group outcomes comparisons had been examined for significance utilizing one-way evaluation of variance (ANOVA) and Fisher’s least vital distinction (LSD) assessments.
Impact of remedy oils on atherosclerotic plaque within the aorta (A) and aortic sinus (B,C). Three mice in every group had been included for evaluation. For B–C, 2 serial sections of every mice had been used, and the imply of two sections from one mice was included within the ultimate evaluation. Atherosclerotic plaque within the aorta was detected by Oil-red O staining (A). Atherosclerotic plaque and lipid deposition within the aortic sinus was detected by H&E (B) and Oil-red O staining (C), respectively. The define of the atherosclerotic lesions within the aortic sinus was marked with a black dashed line. The results of Oil pink O staining within the aortic sinus was normalized by atherosclerotic plaque space. Information had been expressed as imply ± SEM. There was significance if teams didn’t share the identical letter (p < 0.05). CON, well being management; CO, corn oil; FO, fish oil; MO, mussel oil; ASP, aspirin.
Research findings
Comparisons in atherosclerotic plaque space and lesion space between the remedy cohorts utilizing the CON group as a baseline revealed that the CO group was the worst affected. Surprisingly, regardless of slight reductions in lesion space, FO was not discovered to fluctuate from CO outcomes statistically. The MO group was discovered to fare a lot better, with lesion and plaque areas akin to these of fish oASP and CON teams.
Quantification of SMCs revealed comparable tendencies – the variety of SMCs within the MO group was considerably decrease than the CO and FO teams and was akin to ASP outcomes. In distinction, CO and MO cohort outcomes had been statistically indistinguishable in macrophage analyses.
“The MO group had a considerably smaller atherosclerotic plaque space, decrease lipid deposition, decrease contents of easy muscle cell (SMC), and barely decrease contents of macrophage on the aortic sinus than the FO group. Serum concentrations of IL-1β, NF-κB, and VCAM-1 had been comparable within the MO and FO teams and had been considerably decrease than the CO group.”
Intriguingly, the MO group displayed considerably decrease ranges of p65NF-κB, p38MAPK, and VCAM-1 protein expression than FO mice. Given the similarities within the different n-3 PUFA traits of MO and FO, this pathway downregulation suggests itself because the mechanism of motion behind MO’s unexpectedly improved anti-atherosclerosis potential over FO.
Conclusion
The current research reveals that mussel oil considerably outperforms fish oils in anti-atherosclerosis efficacy in murine ApoE−/− mannequin techniques. When mixed with the sustainability of harvesting mussels in comparison with their fish counterparts, this presents the previous as a perfect candidate to interchange the latter in atherosclerosis analysis and future interventions.
Journal reference:
- Li, Okay., Tune, X., Li, H., Kuang, X., Liu, S., Liu, R., & Li, D. (2024). Mussel oil is superior to fish oil in stopping atherosclerosis of ApoE−/− mice. Frontiers in Vitamin, 11, 1326421, DOI – 10.3389/fnut.2024.1326421, https://www.frontiersin.org/articles/10.3389/fnut.2024.1326421/full
